TY  - JOUR
T1  - Nucleotide Sequence, SNPs and Haplotypes among Breeds of Goat Melatonin Receptor <I>1A</I> Gene
AU - Sagawa, Ayane AU - Nomura, Koh AU - Fujii, Akira AU - Kotani, Mai AU - Nagura, Yoshio AU - Sato, Shuji AU - Takahash, Yukimizu AU - Hanada, Hirofumi AU - Kobayashi, Eiji 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 21
SP  - 4022
EP  - 4029
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.4022.4029
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.4022.4029
KW  - Goat
KW  -melatonin receptor
KW  -SNP
KW  -haplotype
KW  -Japan
AB  - Melatonin Receptor 1A (MTNR1A) is a potential candidate gene that may affect seasonal reproduction in goats. The aim of this study was to search for Single-Nucleotide Polymorphisms (SNPs) in the goat <I>MTNR1A</I> gene. Coding exons (1 and 2) were amplified by PCR and sequenced. Sequencing showed that the entire goat MTNR1A coding region consists of 1101 nucleotides and 366 deduced amino acids. The goat MTNR1A (exon 1) nucleotide sequence is most highly homologous to sheep (99.6%) and most distant to mouse and human (77.1 and 85%, respectively). The MTNR1A amino acid sequence is again most similar to sheep (98.6%) and most distant to human and mouse (83.0 and 83.9%). The goat <I>MTNR1A</I> gene differs between Japanese Saanen, Shiba, Boer and three Asian native goats with 18 potential nucleotide substitutions in the coding region and 3 SNPs in the 3' non-coding region. Eleven novel SNPs were detected in this study, adding to 10 SNPs earlir reported. The SNPs were differently distributed among the breeds examined in this study. Haplotype analysis indicated that there are 13 types in the samples and different distributions of any haplotype similar to the SNPs one. Ten SNPs were detected following PCR-RFLP using suitable restriction enzymes. These results provide valuable information on polymorphisms in goat breed genotypes which may be used for further characterization and comparative studies in goat breeds.
ER  -