TY  - JOUR
T1  - Sero-Diagnosis of <I>Fasciola gigantica</I> Infestations in Goats Using Elisa and its Comparison with Fecal Sample Analysis
AU - Kim, Bumseok AU - Islam, Mohammad Rafiqul AU - Taimur, Mahbub Jong Fateh Ali AU - Tanzim, Nivana AU - Sultana, Tazminaz AU - Roh, Yoon-Seok 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 20
SP  - 3806
EP  - 3811
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.3806.3811
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.3806.3811
KW  - Antibody
KW  -egg
KW  -ELISA
KW  -fascioliasis
KW  -goat
KW  -Bangladesh
AB  - Fascioliasis, one of the most economically important helminth 
  infestations of goats worldwide can be diagnosed using fecal examination and 
  ELISA of serum samples. The aims of the present study were to compare ELISA 
  and fecal examination for diagnosis of fascioliasis and to estimate the point 
  prevalence of <I>Fasciola gigantica</I> in goats from two selected farms. Both 
  serum and fecal samples (n = 100) were collected randomly from two goat farms. 
  Antibodies against the f2 antigen of Fasciola were detected using ELISA. Fecal 
  samples were examined using the modified McMaster technique. Among 100 goats, 
  38 serum samples showed the presence of antibody against fasciola and 21 of 
  these had fasciola eggs. Overall, 27% goats were infested with fascioliasis 
  with varying degrees of severity. The 28 sero-positive goats were retested for 
  the presence of eggs and antibody 30 days after treatment with broad-spectrum 
  anthelmintics. No eggs were found in the fecal samples but 24 goats remained 
  sero-positive at varying degrees according to ELISA. The anthelmintics presumably 
  destroyed the existing eggs and larvae but the antibody was still present and 
  only detectable using ELISA. ELISA therefore offers a suitable technique for 
  the rapid, specific and accurate detection of fascioliasis in goats.
ER  -