TY  - JOUR
T1  - Polymorphisms in the Third Exon of <I>GH</I> Gene in Chinese Indigenous 
  Donkey
AU - Zhu, Wenjin AU - Su, Yongmei AU - Liu, Yanfang AU - Ni, Jing AU - Wu, Jianhua 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 20
SP  - 3728
EP  - 3731
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.3728.3731
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.3728.3731
KW  - Donkey
KW  -GH gene
KW  -exon 3
KW  -polymorphism
KW  -PCR-SSCP
KW  -glutamic acid
AB  - To reveal genetic diversity for the breed resource evaluation 
  and utilization of Chinese indigenous donkey, polymorphisms in the third exon 
  of <I>GH</I> gene was analyzed by PCR-SSCP and DNA sequencing methods in 16 
  donkey breeds (DZ, GZ, GL, JN, BH, LX, JM, QY, BY, HY, LZ, HB, GY, YY, TH and 
  XJ). The results showed that there was a transition at nucleotide position 957 
  genomic DNA of <I> GH</I> gene, named as GH-exon3-G957A which led to a glutamic 
  acid to lysine substitution at amino acid position 81. Two different alleles, 
  A and B were identified and three genotypes were observed, AA, AB and BB with 
  the frequency distribution of allele A from 0.6667-0.8333 in the analyzed populations. 
  The genetic diversity analysis revealed that PIC values were between 0.346 and 
  0.491 except JM donkey (0.239), implying that this locus within <I>GH</I> gene 
  possessed a moderate genetic diversity in Chinese indigenous donkeys. The &#967;<SUP>2</SUP>-test 
  showed that LX, GY and TH donkeys were no significant deviation, GZ, JN, QY, 
  HB and XJ donkey were significant deviation, DZ, GL, BH, JM, BY, HY, LZ and 
  YY donkey were very significant deviation. The results confirmed that there 
  were polymorphisms in the third exon of <I>GH</I> gene for the first time.
ER  -