TY - JOUR T1 - The Correlation Between Goat Cox II Gene Single Nucleotide Polymorphism and Sperm Motility AU - Zhang, Xiao-Rong AU - Ling, Yinghui AU - Wang, Lijuan AU - Zhang, Xiaodong AU - Xu, Lina AU - Ding, Jian-Ping AU - Zhang, Yun-Hai AU - Zhang, Zijun JO - Journal of Animal and Veterinary Advances VL - 11 IS - 20 SP - 3677 EP - 3681 PY - 2012 DA - 2001/08/19 SN - 1680-5593 DO - javaa.2012.3677.3681 UR - https://makhillpublications.co/view-article.php?doi=javaa.2012.3677.3681 KW - Goat KW -COXII gene KW -genetic variation KW -sperm motility KW -breeding KW -mutation AB - This study is about the correlation between COX II gene genetic variation and adult buck sperm motility traits, to provide genetics reference for the goat breeding. In this study, 120 adult male goat semen samples were collected from four populations. COX II gene polymorphism information was rapid screened using DNA pool and sequencing method. Each individual polymorphism of these goats was detected by RFLP. Sequencing results of DNA pool showed a single nucleotide mutation in COX II gene (A→G). The mutation caused a change of Hind III restriction site. RFLP results showed that the mutation associated with goat sperm traits. The vitality of fresh sperm and frozen sperm from BB-type goats was significantly higher than AA-type. This study provided foundation for establishment relationship between COX II gene SNP and goat sperm motility traits. It could be considered as a reference marker-assisted selection for buck semen quality traits and goat breeding. ER -