TY  - JOUR
T1  - Rapid Detection of Virulent Salmonella Strains Using Multiplex PCR
AU - Yang, Zengqi AU - Qiu, Li AU - Sun, Chao AU - Yang, Hu AU - Li, Chunli AU - Feng, Zhang AU - Zhao, Yahui AU - Liu, Xiaoye AU - Wang, Rui AU - Duan, Min AU - Wang, Xinglong 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 19
SP  - 3544
EP  - 3549
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.3544.3549
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.3544.3549
KW  - Salmonella
KW  - multiplex PCR
KW  -virulence detection
KW  -strains
KW  -reaction
KW  -China
AB  - This study aims to establish a rapid and sensitive multiplex PCR Method for Salmonella detection and the differentiation of virulent strains. Four sets of primers that target hisJ, spvR and fliC were designed and the PCR reaction conditions were then optimized. Based on the detection of reference Salmonella strains and other reference bacterial genera, the PCR Method was able to detect hisJ, spvR, fliC-c and fliC-i in Salmonella. The minimum detectable DNA concentration of the method is 100 pg. The results using the reference strains were consistent with previous research. In addition, this method can also be used in identifying <I>S. typhimurium</I> and <I>S. arizona</I>. Researchers successfully developed a convenient and useful multiplex PCR Method for detecting Salmonella and monitoring virulent strains.
ER  -