TY  - JOUR
T1  - Associations between Immune Traits and <I>MHC B-F</I> Gene in Shandong Indigenous Chickens
AU - Cao, D.G. AU - Li, F.W. AU - Lu, Y. AU - Lei, Q.X. AU - Zhou, Y. AU - Han, H.X. AU - Li, G.M. AU - Wu, B. AU - Wang, S.B. 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 19
SP  - 3481
EP  - 3485
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.3481.3485
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.3481.3485
KW  - Chicken
KW  -MHC B-F
KW  -PCR-SSCP
KW  -immune traits
KW  -vaccination
AB  - In order to find the relationship between immune traits and the Major Histocompatibility Complex B-F (<I>MHC B-F</I>) gene, an immune traits model was established in Wenshang Barred Chicken (LH), Laiwu Black Chicken (LWH) and Jining Bairi Chicken (BR). PCR-SSCP and sequencing methods were used to identified haplotypes in these three Shandong indigenous chickens. As a result, 53 (LH), 52 (LWH) and 54 (BR) Single Nucleotide Polymorphisms (SNPs) were found in the 264 bp of exon 2 in chicken <I>MHC B-F</I> gene. The least square analysis showed that 2, 2, 3 SNPs were respectively found significant associations with antibody responses to H5, H9 and ND in LH chickens; 1, 3 and 3 SNPs were respectively found significant associations with antibody responses to H5, H9 and ND in LWH chickens but none with SRBC. In BR chickens, there was association with responses to H5 (2 SNPs), H9 (3 SNPs), ND (3 SNPs) and SRBC (3 SNPs). These results indicate that the genomic region bearing exon 2 of the <I>MHC B-F</I> gene has significant effects on antibody responses to SRBC and vaccination against AI and ND.
ER  -