TY  - JOUR
T1  - Comparison of Extraction Methods to Detect Porcine Circovirus-2 and Porcine Reproductive and Respiratory Syndrome Virus in Semen Samples
AU - Kim, Won-Il AU - Cho, Yong-Il AU - Cho, Ho-Seong AU - Shin, Gee-Wook AU - Kwon, Jung-Kee AU - Park, Chul 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 17
SP  - 3175
EP  - 3178
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.3175.3178
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.3175.3178
KW  - Semen
KW  -high-throughput extraction
KW  -porcine circovirus-2
KW  -porcine reproductive
KW  -respiratory syndrome virus
KW  -swine
AB  - Since, Porcine Circo Virus-2 (PCV2) and Porcine Reproductive 
  and Respiratory Syndrome Virus (PRRSV) are shed in semen for a long period of 
  time after infection and artificial insemination is a common practice in pig 
  farms, semen is an important source of spreading these viruses to naive populations. 
  Therefore, rapid and accurate detection of PCV2 or PRRSV in semen could be critical 
  at the standpoint of disease prevention and control. In this study, the performance 
  of three different extraction methods on semen samples was compared to determine 
  an optimal extraction method for semen samples to detect PCV2 or PRRSV. Seven 
  or 115 semen samples were collected from the boars experimentally challenged 
  with PRRSV or PCV2, respectively. These two sets of the semen samples were processed 
  by three different extraction methods: High-Throughput Total Nucleic Acids Isolation 
  kit (HT-TNA), High-Throughput Viral RNA Isolation kit with modified procedure 
  (mHT-VR) and DNeasy kit or RNeasy kit for PCV2 or PRRSV, respectively. Then, 
  the efficiency of the extraction methods were compared by conducting the same 
  real-time PCR for each virus. HT-TNA and mHT-VR kits were faster and more convenient 
  to process semen samples and HT-TNA kit showed higher or compatible sensitivity 
  as compared to the other two methods while all three methods demonstrated 100% 
  specificity. In conclusion, the HT-TNA Extraction Method could significantly 
  reduce testing time and effort to process semen samples and showed better sensitivity 
  for detection of PCV2 and PRRSV in semen.
ER  -