TY  - JOUR
T1  - The Expression Analysis of Ghrelin and Growth Hormone Secretagogue Receptor in Mouse Thymic Epithelial Cells
AU - Li, Yu-Gu AU - Liu, Jian-Hong AU - Ma, Yong-Jiang AU - Li, Ying AU - Peng, Hai-Bin AU - Li, Dao-Tong AU - Zhan, Yuan 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 16
SP  - 3027
EP  - 3030
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.3027.3030
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.3027.3030
KW  - Ghrelin
KW  -GHS-R
KW  -MTEC1 cells
KW  -expression
KW  -prokin
KW  -China
AB  - The present study was to identified the expression of Ghrelin and GHS-R in mouse Medullary-Type Epithelial Cells (MTEC1). Whether the gene of ghrelin and GHS-R are expressing in MTEC1 cells or not was measured by using Quantitative real time RT-PCR (Q-PCR). Results identified that the mRNA of ghrelin and GHS-R can be detected in MTEC1 cells. The protein levels of GHS-R and ghrelin precursor were detected by the method of Western blot analysis. Moreover, the location of GHS-R in MTEC1 cells were detected by immunofluorescence and the quantity of ghrelin in the supernatant of MTEC1 cells were measured by ELISA. The results of Western blot analysis make sure that GHS-R and precursor of ghrelin are expressing in MTEC1 cells. GHS-R is a kind of transmembranes protein. As the results of immunofluorescence shown the quantity of ghrelin in the supernatant of MTEC1 cells measured by ELISA was 39.20 pg mL<SUP>-1</SUP> while the control was 5.74 pg mL<SUP>-1</SUP>.
ER  -