TY  - JOUR
T1  - Tissue Distribution and Relative Quantitation of Experimental Infection with Peste Des Petits Ruminant Virus in Goats Using Real-Time PCR (TaqMan<SUP>&reg;</SUP>)
AU - Cai, Xuepeng AU - Meng, Xuelian AU - Dou, Yongxi AU - Zhai, Junjun AU - Shi, Xiaoni AU - Yan, Fengchao AU - Zhang, Hairui AU - Luo, Xuenong 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 16
SP  - 3011
EP  - 3018
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.3011.3018
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.3011.3018
KW  - Peste des petits ruminant virus
KW  -tissue distribution
KW  -goat
KW  -real-time PCR
KW  -Tymph
KW  -China
AB  - Peste des Petits Ruminants (PPR) is an acute, highly contagious and economically important viral disease of small ruminants, especially goats and sheep. Given the contagious nature of the disease, speed of diagnosis is essential in order to quarantine infected animals and initiate appropriate prophylactic measures. Here researchers reported the distribution of PPRV RNA in various tissues of goats experimentally infected subcutaneously with a vaccine virus (Nigeria 75/1) strain in relation to the time post inoculation (pi) by real-time PCR (rt RT-PCR) based on TaqMan<SUP>&reg;</SUP> and was quantified as a fold change (2<SUP>-&#916;&#916;Ct</SUP> values) compared with that of blood at 3 dpi. PPRV levels at 3, 5 and 28 dpi were higher than those at the other time points, especially at 5 dpi. The PPRV level in thymus, tonsil, hilar lymph node and turbinate bone was higher than that of other tissues. The result suggested that the distribution of PPRV in goats were similar to that of viral antigens and rt RT-PCR was useful for the detection of PPRV in field samples. The higher levels of RNA detected at 28 dpi may suggest some level of persistent infection. Furthermore, it was shown that blood sample was the most valuable diagnostic material for use in an epidemic situation.
ER  -