TY  - JOUR
T1  - Sex Determination in Ovine Embryos Using Amelogenin (<I>AMEL</I>) Gene by High Resolution Melting Curve Analysis
AU - Yang, B.H. AU - Yue, Yao Jing AU - Liu, Jian Bin AU - Guo, Ting Ting AU - Feng, Rui Lin AU - Guo, Jian AU - Sun, Xiao Ping AU - Niu, Chun E. 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 16
SP  - 2948
EP  - 2952
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.2948.2952
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.2948.2952
KW  - B.H. Yang
KW  -amelogenin gene
KW  -sexing
KW  -high-resolution melting
KW  -blood
KW  -China
AB  - In the study, researchers have established and tested the reliability of a method for sex determination of ovine embryos using the <I>AMEL</I> gene by melting curve analysis of PCR amplification. It can be carried out in a regular laboratory or under farm conditions within 1.5 h for 96 samples. The PCR amplicons of 99/99 and 99/54 base pairs produced from female and male sheep, respectively are easily distinguished by both melting curve analysis and gel electrophoresis. The specificity of the method was earlier demonstrated by testing 9 blood samples from small-tailed sheep (5 males and 4 females). No amplification failures and very high agreement between genotypic and phenotypic sex was found (9/9). The sensitivity of the AMEL sexing assay was established for values &gt;10 pg ovine genomic DNA. Forty five biopsied embryos were transferred into 22 recipient sheep on the same day that the embryos were collected and sex of the kid was confirmed after parturition. About 17 kids of predicted sex were born. The sex, as determined by PCR corresponded to the anatomical sex in all cases. To the knowledge, this was the first time that sex determination using the amelogenin gene was performed in ovine embryos by melting curve analysis.
ER  -