TY  - JOUR
T1  - Factors Affecting the Derivation and Expansion of Bovine Embryonic Stem Cell-Like Cells
AU - Wang, Rui AU - Cong, Shan AU - Cang, Ming AU - Ma, Yuzhen AU - Wen, Jianxun AU - Liu, Dongjun 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 16
SP  - 2865
EP  - 2872
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.2865.2872
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.2865.2872
KW  - Bovine embryonic stem cell-like cells
KW  -MEF
KW  -feeder layer density
KW  -IGF
KW  -blastocysts
AB  - Bovine ES cells have been isolated but validated bovine ES cell lines have not yet been successfully established. Here, researchers investigated the factors that affect the derivation, proliferation and maintenance of bovine Embryonic Cell (bESC)-like cells including type of feeders (Mouse Embryonic Fibroblasts (MEFs) vs. Bovine Embryonic Fibroblasts (BEFs)), feeder density and inner cell mass isolation procedure. We found that the MEFs work better than BEFs on the process and best at a cell density of 1.25x10<SUP>5</SUP> cells/cm<SUP>2</SUP>, naturally hatched blastocysts had a short adherence time and high rate of adherence. They were more conducive to bESC-like cell isolation and culture. Culture medium supplemented with IGF promoted proliferation of bESC-like cells but was unfavorable for the maintenance of pluripotency. The findings provide information on the establishment of culture systems for bESC-like cells.
ER  -