TY - JOUR T1 - Deep-Sequencing Analysis of DF-1 Cell Transcriptome Response to Infection with Newcastle Disease Virus of Duck AU - Ren, T. AU - Gao, Shi-Min AU - Sun, Min-Hua AU - Wang, Zhao-Xiong AU - Li, Xian-Wei AU - Fan, Hui-Ying JO - Journal of Animal and Veterinary Advances VL - 11 IS - 14 SP - 2556 EP - 2563 PY - 2012 DA - 2001/08/19 SN - 1680-5593 DO - javaa.2012.2556.2563 UR - https://makhillpublications.co/view-article.php?doi=javaa.2012.2556.2563 KW - Digital Gene Expression (DGE) KW -NDV-YC KW -pathway KW -GO analysis KW -symptoms AB - Newcastle Disease (ND) is caused by Newcastle Disease Virus (NDV) with a respiratory and digestive mucosal bleeding for typical symptoms. It is an acute highly contacting disease. NDV could multiplicate in DF-1 cells. NDV from waterfowl is different from former NDV from chicken, it lead waterfowl dead. But the molecular mechanisms of waterfowl NDV infected still are poorly understood. In this study researchers employed the illumina genome analyzer platform to perform genome-wide Digital Gene Expression (DGE), a tagbased high-throughput transcriptome sequencing method analysis of DF-1 cells infected NDV-YC. Researchers could reach a sequencing depth of 5.0-6.1 million tags per library and found >8000 genes to be differentially expressed during NDV-YC infection processes. NDV-YC notably identified genes involved in I-kappaB kinase/NF-kappaB and TGF-beta signaling pathway. Further analysis employed Gene Ontology (GO) analysis cell part, binding and biological regulation terms are main items, respectively. The data benefit for better understanding the molecular mechanism of NDV-YC infected and provide a basis for further in vivo study and clinical trials. ER -