TY  - JOUR
T1  - Protein Changes in Longissimus Dorsi Muscle of Chinese Yellow Crossbred Bulls 
  as a Result of Electrical Stimulation
AU - Luo, Xin AU - Shen, Jin AU - Li, Nan AU - Mao, Yanwei AU - Zhang, Yimin 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 13
SP  - 2301
EP  - 2306
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.2301.2306
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.2301.2306
KW  - Chinese yellow crossbred bulls
KW  -muscle protein changes
KW  -beef quality
KW  -electrical stimulation
KW  -proteolysis
KW  -two-dimensional gel electrophoresis
AB  - Protein changes induced by low-voltage Electrical Stimulation (ES; 42V for 40 sec) in the insoluble protein fraction of bovine Longissimus Dorsi (LD) muscle at 3 days post-ES were investigated by proteomics. Protein abundance patterns from ten Chinese yellow crossbred bulls were compared and significant changes due to ES were found. Seven protein spots showed lower expression abundance in 3 days post-ES samples including myofibrillar and cytoskeletal protein (myosin binding protein H, histone H3.3-like isoform 2) two metabolic enzymes (creatine kinase, triosephosphate isomerase) and an unnamed protein due to ES. Reduced abundance of these proteins in ES samples indicated an accelerated proteolysis due to ES which finally improves tenderness. The most important finding was that an unnamed protein product was found to change in abundance due to ES at 3 days post-ES and this protein could provide a new target as a potential meat quality biomarker. These findings provided a better understanding of the biochemical processes taking place as a result of ES during postmortem storage of meat.
ER  -