TY  - JOUR
T1  - Culture of Chicken Gonadal Primordial Germ Cells (gPGCs) in Chicken Embryonic 
  Fibroblast (CEF) Cells Conditioned Medium and<I> in vivo </I>Migration
AU - Chang, Ming-Cheng AU - Liou, Jenn-Fa AU - Shiau, Jen-Wen AU - Tailiu, Jui-Jane AU - Tai, Chein AU - Chen, Lih-Ren 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 13
SP  - 2196
EP  - 2203
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.2196.2203
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.2196.2203
KW  - Chicken
KW  -Chicken Embryonic Fibroblast (CEF)
KW  -gPGCs
KW  -migration
KW  -Schiff reaction
AB  - The primitive gonads collected from 5.5 days old chicken embryos were dissociated and explanted onto plates pre-coated with 0.1% gelatin. The conditioned media from proliferating and Chicken Embryonic Fibroblast (CEF) cells was supplemented with growth factors and used to support the growth of gPGCs. The result showed that the conditioned media could promote the growth and colony formation of gPGCs <I>in vitro</I> in particular the conditioned by CEF cells. The gPGC derived colonies maintained in CEF cells-conditioned medium up to 281 days were positively antibodies specific to anti-SSEA-1, SSEA-4 integrin &#945;6 and integrin &#946;1 and stained by periodic acid Schiff reaction. Their capacities of migration via vascular system and taking up residence in the primary gonadal ridge were further demonstrated by transferring to the dorsal aorta of stage 17 recipient embryos. These results suggested that the culture system is able to maintain chicken gPGCs for long-term <I>in vitro</I> culture without losing their capacity to express pluripotent markers and to integrate into the gonads.
ER  -