TY  - JOUR
T1  - Identification of Intrauterine Mycoplasma in Endometritis of Dairy Cow Isolates by 16S rDNA Nested PCR-DGGE
AU - Guo, Meng-Yao AU - Wang, Guo-Qing AU - Shao, Guoxi AU - Tian, Wulin AU - Xu, Yuanjing AU - Xie, Guanghong 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 10
SP  - 1745
EP  - 1752
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.1745.1752
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.1745.1752
KW  - Mycoplasma
KW  -endometritis
KW  -cultures
KW  -cow`s reproductive system
KW  -China
AB  - Molecular diagnostics methods are used to investigate mycoplasma infection status but this approach to infection analysis of mycoplasma in endometritis of dairy cow has not been reported. In this study, a nest Polymerase Chain Reaction and Denaturing Gradient Gel Electrophoresis (nested PCR-DGGE) assay for detection of 16S ribosomal RNA of mycoplasma was used as reference of 4 isolates which were identified in a previous survey of dairy cow’s reproductive system in Jilin province, China. A reference PCR-DGGE profile was generated using DNA extracted and amplified from the strain of these cultures. The effectiveness of the procedure was assessed by application to test the samples from endometritis dairy cow’s uterus mucus. The bands from the nested PCR-DGGE were extracted for sequencing.
ER  -