TY  - JOUR
T1  - Tissue Expression Level Analysis of CD14 in Buffalo
AU - , Fengyang Wang AU - Zhang, Donglin AU - , Yongchang Hao AU - , Ying Cheng AU - , Li Du AU - , Ming Lei AU - , Hanwei Jiao AU - , Jianing Zhang AU - , Hui Rong AU - , Xiaoxiao Jia 
JO  - Journal of Animal and Veterinary Advances
VL  - 11
IS  - 9
SP  - 1353
EP  - 1355
PY  - 2012
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2012.1353.1355
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2012.1353.1355
KW  - Buffalo
KW  -CD14
KW  -real-time RT-PCR
KW  -Western blot
KW  -tissue expression level
KW  -China
AB  - Brucellosis is an extremely important disease affecting the health of human and a number of animal species (including buffalo) around the world and <I>Brucellae</I> sp. is the causative agent of brucellosis. As an important innate immunity molecule, CD14, involved in protecting a host against invading microorganism including Brucella. To analyze the expression of CD14 and provide new alternatives for elucidating the molecular mechanism of Brucella infection in bufflo, real-time quantitative Reverse Transcription PCR (RT-PCR) and western blot were performed to assess the mRNA level and protein levels of CD14 in heart, liver, spleen, lung, kidney and intestine. The results indicated that CD14 mRNA and protein are ubiquitously present in all buffalo tissues examined with relatively high levels in lung and relatively low levels in intestine. These data provide important anatomical information for studying the role of CD14 played in Brucella infection in the different tissue of buffalo.
ER  -