TY  - JOUR
T1  - Effects of Ghrelin on <I>in vitro</I> Nuclear Maturation and Subsequent Embryo Development of Immature Bovine Oocytes
AU - Dashtizad, M. AU - Wahid, H. AU - Mazni, O. Abas AU - , Y. Rosnina AU - , M. Daliri AU - , H. Hajarian 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 21
SP  - 2797
EP  - 2801
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.2797.2801
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.2797.2801
KW  - Ghrelin
KW  -nuclear maturation
KW  -bovine oocyte
KW  -in vitro embryo development
KW  -bovine
AB  - Development of efficient culture system to support embryonic development would be valuable when percentage of produced embryos reaching to the blastocyst stage is important. However, the rate of bovine embryo production <I>in vitro </I>is still lower than expected. Present study was performed to investigate the effect of ghrelin on nuclear maturation and subsequent bovine embryo development <I>in vitro</I>. Cumulus-oocyte-complexes were collected from slaughterhouse ovaries and randomly allocated in each treatment groups. Five different concentrations of ghrelin (0, 5, 50, 500 and 1000 ng mL<SUP>-1</SUP>) were added to the <I>in vitro </I>maturation medium (Hepes-buffered medium 199+fetal calf serum+gonadotrophins+insulin+antibiotics). The proportion of oocytes developed to metaphase II stage was significantly increased at 5 and 50 ng mL<SUP>-1</SUP> ghrelin (86.32&plusmn;3.38 and 89.77&plusmn;2.92%, respectively). The result also indicated that adding high concentration of ghrelin adversely affect (p&lt;0.05) the nuclear maturation rates of bovine oocytes. However, the subsequent embryo development was not significantly affected by addition of ghrelin to the IVM medium. This study showed that inclusion of 5-50 ng mL<SUP>-1</SUP> ghrelin in maturation medium may have beneficial effects on nuclear maturation of bovine oocytes <I>in vitro</I>.
ER  -