TY - JOUR T1 - The Distributions of S. enteritidis in the Internal Organs of Chinese Guizhou Minipig after Orally Infected AU - Qian, Ning AU - Tian, Wei-Yi AU - He, Guang-Zhi AU - Deng, Shu-Xuan JO - Journal of Animal and Veterinary Advances VL - 10 IS - 17 SP - 2275 EP - 2277 PY - 2011 DA - 2001/08/19 SN - 1680-5593 DO - javaa.2011.2275.2277 UR - https://makhillpublications.co/view-article.php?doi=javaa.2011.2275.2277 KW - China KW -infection sites KW -PCR KW -enteritidis KW -quantitative study KW -mini-pig AB - Chinese Guizhou mini-pigs were orally infected with a high-virulence strain of S. enteritidis. The kinetics of the S. enteritidis genomic deoxyribonucleic acid loads was analysised by the real-time PCR assay and the histopathological examination in various tissues were investigated. The results showed that at 4 h postinoculation, high S. enteritidis deoxyribonucleic acid loads were observed in various organs of the infected pigs. Thereafter, the bacterial deoxyribonucleic acid loads increased by various amounts until 2nd day postinoculation. The time course of the appearance of bacterial antigens and tissue lesions in various tissues was coincident with the levels of the bacterial deoxyribonucleic acid loads at the infection sites. ER -