TY  - JOUR
T1  - Populations of <I>Salmonella enteritidis</I> in Orally Infected White Chinese Goose
AU - Deng, Shu-Xuan AU - Qian, Ning AU - Tian, Wei-Yi AU - He, Guang-Zhi AU - Feng, Yong AU - An, Chuan-Wei 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 17
SP  - 2234
EP  - 2239
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.2234.2239
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.2234.2239
KW  - detection
KW  -China
KW  -goose
KW  -internal organs
KW  -distribution
KW  -Salmonella enteritidis
KW  -tissues
AB  - The objective of this study was to understand populations of <I>Salmonella enteritidis</I> (<I>S. enteritidis</I>) in the internal organs of geese after oral challenge. Researchers conducted serovar-specific Fluorescent Quantitative Real-Time PCR (FQ-PCR) for <I>S. enteritidis</I> to detect the genomic DNA of <I>S. enteritidis</I> in the blood and the internal organs including heart, liver, kidney, duodenum, jejunum, ileum, cecum, the bursa of fabricius, thymus, spleen and harderian gland from geese after oral challenge at different time points. To validate these results, the Indirect Fluorescent Antibody (IFA) technique was employed. The results showed that <I>S. enteritidis</I> was consistently detected in all the samples. Ileum and jejunum were positive at 8 h PI and the last organ to show a positive result was the thymus at 36 h PI. The copy numbers of <I>S. enteritidis</I> DNA in each tissue reached a peak at 24 h and 2nd day PI with the blood, jejunum, ileum, liver and spleen containing higher concentrations than other tissues. However, the number of bacteria started decreasing by 2-3 days and by 6 days, the concentration of <I>S. enteritidis </I>DNA was below the detection limits of the FQ-PCR assay except the spleen. The IFA results were similar to those of the FQ-PCR. This study will help in understanding the pathogenesis of <I>S. enteritidis</I> infection <I>in vivo</I>.
ER  -