TY  - JOUR
T1  - Comparative Analysis of 18S and 28S rDNA Sequences of <I>Schistosoma japonicum</I> from Mainland China, the Philippines and Japan
AU - Zhao, G.H. AU - Zou, F.C. AU - Lin, R.Q. AU - Weng, Y.B. AU - Sugiyama, H. AU - Chen, F. AU - Li, J. AU - Song, H.Q. AU - Zhu, X.Q. AU - Yuan, Z.G. 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 15
SP  - 2010
EP  - 2015
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.2010.2015
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.2010.2015
KW  - phylogenetic analysis
KW  -18S rDNA
KW  -28S rDNA
KW  -Philippines
KW  -mainland China
KW  -Schistosoma japonicum
KW  -Japan
AB  - In the present study, a portion of the 18S and 28S ribosomal DNA (rDNA) sequences of 35 <I>Schistosoma japonicum</I> isolates representing three geographical strains from mainland China, the Philippines and Japan were amplified and compared and phylogenetic relationships were also reconstructed by Unweighted Pair-Group Method with Arithmetic averages (UPGMA) using combined 18S and 28S rDNA sequences as well as the corresponding sequences of other species belonging to the Schistosoma genus available in the public database. The results indicated that the partial 18S and 28S rDNA sequences of all <I>S. japonicum</I> isolates were 745 and 618 bp, respectively and displayed low genetic variation among <I>S. japonicum</I> strains and isolates. Phylogenetic analysis revealed that the combined 18S and 28S rDNA sequences were not able to distinguish <I>S. japonicum</I> isolates from three geographical origins but provided an effective molecular marker for the inter-species phylogenetic analysis and differential identification of different <I>Schistosoma</I> species.
ER  -