TY  - JOUR
T1  - Study on the Resistance of Extended Spectrum β-Lactamases
AU - Wang, Jian-Hua AU - Shen, Yong-Shu AU - Zhang, Xiao-Gen AU - Zhang, Chun-Hui 
JO  - Journal of Animal and Veterinary Advances
VL  - 10
IS  - 8
SP  - 1032
EP  - 1036
PY  - 2011
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2011.1032.1036
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2011.1032.1036
KW  - detection
KW  -multi-drug resistance
KW  -E. coli
KW  -ESBLs
KW  -elimination
KW  -China
AB  - The multi-drug resistance of bacteria is a serious threat to the treatment of clinical infectious diseases; <I>Escherichia coli</I> (<I>E. coli</I>) possess plasmid-encoded Extended Spectrum &#946;-Lactamases (ESBLs) that confer multi-drug resistance. In order to overcome this kind of resistance, some experiments were done in this study. In this study for the clinic pathogenic <I> E. coli</I>, ESBLs genetype were detected with PCR and for the induced <I>E. coli</I>, the plasmid elimination effects of several plasmid-curing agents to ESBLs were investigated using SDS as a control. Results showed that the bacteria producing ESBLs were amplified TEM type ESBLs fully, two strains were amplified both TEM type ESBLs and SHV type ESBLs, only one strain was amplified both TEM type ESBLs and CTX type ESBLs. And to the induced <I>E. coli</I> C83845-y, the elimination rate of chlorpromazine, hydrochloride, diazepam, norfloxacin and ciprofloxacin was 14.53, 13.50, 14.59, 8.43 and 8.23%, respectively, the elimination rate of SDS was 11.05%, blank control was 0.05% to induce <I>E. coli</I> C83907-y, the elimination rate of chlorpromazine, hydrochloride, diazepam, norfloxacin and ciprofloxacin was 11.25, 12.23, 13.51, 9.91 and 9.03%, respectively, the elimination rate of SDS was 10.72%, blank control was 0.05%. These results indicated that ESBLs-positive <I>E. coli</I> in Henan have transmitted resistant gene plasmids among different bacteria, the tricyclic-psychotropic drugs were more effective at eliminating the ESBL-resistant plasmid. This study provides insight into strategies for overcoming the resistance conferred by ESBLs.
ER  -