TY  - JOUR
T1  - Role of Flagella-Related <I>rPoN</I> and <I>fliA</I> Genes in <I>Campylobacter jejuni</I>
AU - Lee, Young Ju AU - Ku, Bok Kyung AU - Kim, Jong Wan AU - Chae, Myeong Ju AU - Hwang, Jei-Kiun 
JO  - Journal of Animal and Veterinary Advances
VL  - 9
IS  - 24
SP  - 3034
EP  - 3038
PY  - 2010
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2010.3034.3038
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2010.3034.3038
KW  - Campylobacter jejuni
KW  -rpoN
KW  -fliA
KW  -flagella
KW  -Republic of Korea
KW  -colonization
AB  - To investigate the role of the rpoN and fliA in the regulation of <I>C. jejuni</I> colonization, the <I>rpoN</I> and <I>fliA</I> gene were inactivated by allelic exchange with a defective copy of the gene carrying a tetracycline-resistance cassette. Phenotypically, the<I> C. jejuni </I>rpoN mutant exhibited aflagellar filament on transmission electron microscopy and non motility on MH medium supplemented with 0.4% Bacto agar while the fliA mutant possessed a truncated flagellar filament in most cells and strongly diminished motility. Adhesion and invasion ability tests of the rpoN and fliA mutants were performed to assess the role of the these genes in regulating <I>C. jejuni</I> infection. The rpoN and fliA mutants showed significantly less (p&lt;0.0005) adherence and invasion compared to the wild type <I>C. jejuni</I>. To determine the colonization capacity of <I>C. jejuni</I> rpoN and fliA mutants, 1 day old chicks were inoculated with the defined <I>C. jejuni</I> mutants. The <I>C. jejuni</I> rpoN and fliA mutants demonstrated a marked impairment in their ability to colonize chicks as none of 10 chickens inoculated with rpoN and fliA mutants were colonized. In conclusion control of flagellin expression in <I>C. jejuni</I> involves the alternative sigma factor genes <I>rpoN</I> and <I>fliA</I>. The results of this study are consistent with the previous results that both <I>fliA </I>and <I>rpoN</I> genes regulate the process of binding and internalization of <I>C. jejuni</I> to epithelial cells <I>in vitro</I> and <I>in vivo</I>.
ER  -