TY - JOUR T1 - Streptococcus agalactiae the Etiological Agent of Mass Mortality in Farmed Red Tilapia (Oreochromis sp.) AU - Abuseliana, Ali AU - Daud, Hassan AU - Aziz, Saleha Abdul AU - Bejo, Siti Khairani AU - Alsaid, Milud JO - Journal of Animal and Veterinary Advances VL - 9 IS - 20 SP - 2640 EP - 2646 PY - 2010 DA - 2001/08/19 SN - 1680-5593 DO - javaa.2010.2640.2646 UR - https://makhillpublications.co/view-article.php?doi=javaa.2010.2640.2646 KW - Bacteriology KW -identification KW -Oreochromis sp KW -Streptococcus agalactiae KW -warrants KW -kanamycin AB - Streptococcal infection was reported to cause significant mortality and high economical loss in freshwater and saltwater fish species including tilapia species, worldwide. Recently, few disease outbreaks affecting cultured red tilapia in Selangor state, Malaysia was investigated. Affected fish showed loss of appetite, serpentine swimming and exophthalmia. Sick and healthy appeared fish were clinically examined and samples from brain, liver, spleen and kidney were collected for agent isolation. All isolates were gram-positive, oxidase-negative, catalase-negative, β-haemolytic cocci and were characterized as a Group B Streptococcus agalactiae (GBS) using commercial identification kits (Streptococcal grouping Kit, RapID™ STR System and BBL Crystal GP ID Kit). The isolates were sensitive to amoxicillin, ampicillin, erythromycin, chloramphenicol, linomycin, rifampicin, vancomycin, gentamicin, sulfamethoxazole + trimethoprime and tetracycline. In contrast, they were resistant to neomycin, amikacin, kanamycin and streptomycin. Specific Polymerase Chain Reaction (PCR) and 16S rRNA sequencing technique results confirmed the isolates as a GBS. Results of this current study indicated that the Streptococcus agalactiae infection started to spread and warrants focusing on ways to prevent and control the disease before it become endemic. ER -