TY  - JOUR
T1  - Comparison of DNA Polymorphism of Bovine Pituitary-Specific Transcription Factor and Leptin Gene Between Iranian <I>Bos indicus</I> and <I>Bos taurus</I> Cattle Using PCR-RFLP
AU - Nahavandi, Reza AU - Asadzadeh, Nader AU - Soleimani Farjam, Adbdolreza AU - Amin, S.M. Nurul AU - Hafezamini, Parivash AU - Javanmard, Arash 
JO  - Journal of Animal and Veterinary Advances
VL  - 9
IS  - 11
SP  - 1660
EP  - 1663
PY  - 2010
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2010.1660.1663
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2010.1660.1663
KW  - Pit-1
KW  -leptin
KW  -Bos taurus
KW  -Bos indicus
KW  -PCR-RFLP
KW  -polymorphism
AB  - Variations at DNA level contribute to the genetic characterization of livestock populations and this may help to identify possible hybridization events as well as past evolutionary trends. The leptin and Pit-1 are attractive candidate genes for production and reproduction traits in cattle. A total of 247 animals from four breeds from two species of Iranian cattle populations in include <I>Bos taurus</I> (Sarabi, Golpayegani) and <I>Bos indicus</I> (Sistani, Taleshi) were genotyped for the Pit-1 HinfI and leptin Sau3AI polymorphisms by the Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP). The genotype and gene frequencies for each group were determined and shown to be quite variable among the breeds. The allele B for the leptin gene and allele A for the Pit-1 gene were investigated high frequency in<I> Bos indicus</I>. Candidate gene approach may be a useful method to measure of genetic distance for cross breeding program between taurin and indicine cattle.
ER  -