TY  - JOUR
T1  - The Effect of Linear Versus Circular Vector on Enhanced Green Fluorescent Protein (EGFP) Expression in Transgenic Zebrafish (<I>Danio rerio</I>)
AU - Ekici, Aygul AU - Aktoprakligil, Digdem AU - Timur, Metin AU - Akkoc, Tolga AU - Bagis, Haydar 
JO  - Journal of Animal and Veterinary Advances
VL  - 9
IS  - 8
SP  - 1232
EP  - 1236
PY  - 2010
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2010.1232.1236
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2010.1232.1236
KW  - Transgenic fish
KW  -transgenic technology
KW  -fertilized fish egg
KW  -microinjection
KW  -Aequorea victoria
KW  -Danio rerio
AB  - Green Fluorescent Protein (GFP) has been used as an indicator of transgene expression in living cells and organisms. In this study, a transgene construct containing the Cyto-Megalo-Virus (CMV) promoter sequences, SV40 polyA signal and the Enhanced Green Fluorescent Protein reporter gene (EGFP) was microinjected into the cytoplasm of one-cell zebrafish embryos. About 65 ng &#956;L<SUP>-1</SUP> circular and linearized pEGFP-N1 DNA was used in microinjection. Transgenic founders were detected by Polymerase Chain Reaction (PCR), Slot and Southern blots and Reverse-Transcriptase PCR (RT-PCR). EGFP gene expression was detected by inverted fluorescence microscope in F<SUB>0</SUB> transgenic zebrafish larvae. About 54 and 25 F<SUB>0</SUB> transgenic zebrafish were obtained after microinjection of linearized and circular gene constructs, respectively. This is the first study for generation of transgenic zebrafish via cytoplasmic DNA microinjection in Turkey. In conclusion, these results indicate that the gene expression efficiency of circular form was higher than the linearized form in F<SUB>0</SUB> transgenic zebrafish larvae.
ER  -