TY  - JOUR
T1  - Organogenesis and Enzymatic Functionality of Exocrine Pancreas in Cultured Gilthead Sea Bream (Sparus aurata) Larvae
AU - Okan Kamaci, H. AU - Suzer, Cuneyt AU - Coban, Deniz AU - Firat, Kursat AU - Saka, Sahin 
JO  - Journal of Animal and Veterinary Advances
VL  - 8
IS  - 12
SP  - 2477
EP  - 2484
PY  - 2009
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2009.2477.2484
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2009.2477.2484
KW  - Sparus aurata
KW  -exocrine pancreas
KW  -organogenesis
KW  -histology
KW  -enzymes
KW  -growth
AB  - The ontogeny of the exocrine pancreas was studied histological with the expression of digestive protease, trypsin, activity in gilthead sea bream, <I>Sparus aurata</I>, L. larvae from hatching to 30 days after Hatching (DAH). The pancreas was identified as a well developed differentiated cells located dorsal and slightly posterior to the liver at 3 DAH. Incipient pancreas with exocrine polyhedral cells could be observed and first zymogen granules detected at this period. Until the larval metamorphosis, the pancreas became diffuse, spreading throughout the mesentery enclosure the stomach, the upper intestine and the pyloric caeca. The specific activity of trypsin (48.35±4.3 mU/mg/protein) and chymotrypsin (205.38±46.2 mU/mg/protein) was determined as early as after hatching at 2.27±0.14 mm Total Length (TL) of larvae and increased immediately during the following days especially, after exogenous feeding. The highest trypsin and chymotrypsin activity was detected at 25 DAH as 118.26±10.23 mU/mg/protein and 1067.53±118.42 mU/mg/protein, respectively that concurrently detected with <I>Artemia</I> metanauplii introduction. It is concluded that exocrine pancreas organogenesis is the main critical step of the zymogen granules and specific activities digestive proteases, trypsin and chymotrypsin, were present as early as after hatching and continuously increasing with larval period of <I>Sparus aurata</I>.
ER  -