TY  - JOUR
T1  - Correlation Analysis Between Single Nucleotide Polymorphisms in Exon 2 of ADSL Gene and Inosine Monophosphate Acid Content in Chicken
AU - Wu, X.S. AU - Bao, W.B. AU - Shu, J.T. AU - Xu, Q. AU - Zhang, X.Y. AU - Han, W. AU - Chen, G.H. 
JO  - Journal of Animal and Veterinary Advances
VL  - 8
IS  - 12
SP  - 2438
EP  - 2441
PY  - 2009
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2009.2438.2441
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2009.2438.2441
KW  - ADSL gene
KW  -SNP
KW  -inosine monophosphate content
KW  -chicken
KW  - genotype
KW  -Hardy-Weinberg equilibrium
AB  - This study was designed to investigate the effect of Adenylosuccinate Lyase (ADSL) gene on Inosine Monophosphate content (IMP) in chicken. The chickens used for the study included exogenous Recessive White chicken and Chinese indigenous chicken breeds such as Silkies, Baier, Tibetan and Xiaoshan chickens. A pair of primer was designed to detect exon 2 of ADSL gene by PCR-SSCP and mutation detected was directly sequenced. A synonymous mutation at 3484 bp in exon 2 was substituted C/T SNP. In five chicken breeds, three genotypes TT, CT and CC were observed in exon 2. Muscle IMP content of individuals with TT genotype in exon 2 had significantly higher than those with CT and CC genotypes (p<0.05). In exon 2, Recessive White, Xiaoshan and Baier chickens were in Hardy-Weinberg equilibrium (p>0.05) and Silkies and Tibetan chickens were significant departure from Hardy-Weinberg equilibrium (p<0.01).
ER  -