TY  - JOUR
T1  - Expression of Infectious Bovine Rhinotracheitis Virus Glycoprotein B in Bacterial Cell
AU - Momtaz, Hassan AU - Abbasian, Behnam 
JO  - Journal of Animal and Veterinary Advances
VL  - 8
IS  - 9
SP  - 1735
EP  - 1739
PY  - 2009
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2009.1735.1739
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2009.1735.1739
KW  - IBR virus
KW  -gB protein
KW  -pGEX-4T-3 vector
KW  -protein expression
KW  -SDS-PAGE
KW  -immunoblotting
AB  - Bovine Herpesvirus-1 (BHV-1) belongs to the genus of varicellovirus and the family of herpesviridae, which contains three main gB, gC and gD genes and can cause different respiratory, reproductive and nervous system disorders in cows. In order to cloning of the coding region of gB gene of IBR virus, PCR product of the open reading frame of the gene from IBR-MDBK cell line was amplified by PCR. A 723 bp PCR product of the gB gene with <I>EcoRI, SalI </I>restriction sites were subcloned of pTZ57R/T and digested by the mentioned endonucleases. Digested insert cloned into pGEX-4T-3 and transfected in <I>E. coli</I> cells. For the expression of gB protein, the pGEX-4T-3 recombinant vector was transformed and then induced in BL21 (DE3) strain of <I>E. coli</I> competent cells using IPTG, the presence of gB expressed protein was shown in immunoblotting and SDS-PAGE system. With respect to the remarkable frequency of infection to IBR in Iran and the necessity of controlling it through vaccination with recombinant vaccines of thymidine kinase, manufacturing and applying the recombinant gB protein are vital goals in recognition and distinction between infection and responses caused by vaccine.
ER  -