TY - JOUR T1 - Mutation in Exon 4 of apoVLDL-II Gene is a Candidate for Meat Tenderness in Chicken AU - , Wu Sheng-Long AU - , H.H. Musa AU - , Bao Wen-Bin AU - , Wang Ke-Hua AU - , Zhu Guo-Qian AU - , Chen Guo-Hong JO - Journal of Animal and Veterinary Advances VL - 7 IS - 12 SP - 1624 EP - 1627 PY - 2008 DA - 2001/08/19 SN - 1680-5593 DO - javaa.2008.1624.1627 UR - https://makhillpublications.co/view-article.php?doi=javaa.2008.1624.1627 KW - Mutation KW -apoVLDL-II gene KW -meat tenderness AB - The aim of this experiment was to investigate the effect of mutation in apoVLDL-II (apo very low density lipoprotein II) gene on meat quality traits of genetically fat (Anka) and lean (Rugao) chicken breeds. Meat quality traits such as Water Holding Capacity (WHC), pH, Color Density (CD) and tenderness (Shear value (kg cm 2)) were analyzed from breast muscle. Polymerase chain reaction single strand conformation polymorphism (PCR-SSCP) technique was developed to analyze a 280 bp region of the apoVLDL-II gene (exon 4). The average gene frequency of two breeds estimated was found to be 0.076±0.008 for allele A and 0.932±0.008 for allele B. The effective number of allele, gene diversity and Shannon`s Information index were 1.1642±0.0195, 0.1408±0.0144 and 0.2691±0.0212, respectively. Mutation in the apoVLDL-II gene was found to significantly (p<0.05) affect meat tenderness, whereas no significant effect was observed on OD, pH and WHC. ER -