TY  - JOUR
T1  - Characterization of Microvesicles of <I>Mannheimia haemolytica</I> Serotype A1 (Reference Strain) and Serotype A2 (Field Isolate)
AU - , Cynthia Gonzalez-Ruiz AU - , Victor Tenorio-Gutierrez AU - , Francisco Trigo-Tavera AU - , Magda Reyes-Lopez AU - , Nidia Leon-Sicairos AU - , Delfino Godinez-Vargas AU - , Mireya de la Garza-Amaya 
JO  - Journal of Animal and Veterinary Advances
VL  - 6
IS  - 10
SP  - 1172
EP  - 1182
PY  - 2007
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2007.1172.1182
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2007.1172.1182
KW  - Microvesicles
KW  -Mannheimia haemolytica
KW  -ovine
KW  -serotype
KW  -LPS
AB  - <I>Mannheimia haemolytica</I> is a commensal of cattle, sheep and other ruminants, but it can also cause bovine and ovine pneumonic Pasteurellosis (infecting the respiratory tract), which is responsible for considerable economic losses in cattle and sheep industries. <I>M. haemolytica</I> shows different antigens. Each one of these antigens could also contribute to the development of disease and induce a specific immune response by the host. Gram negative bacteria contain outer membrane vesicles that are secreted into the medium under certain growth conditions. These vesicles retain outer membrane and cell wall constitutive components such as proteins, Lipo-Polysaccharides (LPS), muramic acid, capsule, fimbria and others. These components play a crucial role in the development of the illness as well as in the immune response activation. Antigens of immunological interest could be inside of the small Microvesicles (MVs) released by <I>M. haemolytica</I> under stress conditions. The immunogenicity of these MVs could stimulate an adequate humoral response, in the defense of individuals exposed to virulent strains and prevent animals from developing pathological signs. The importance of obtaining an effective immunogen lays in the prevention and control of pneumonic ovine Pasteurellosis. The objective of this study was to obtain and characterize antigens from Microvesicles (MVs) of <I>Mannheimia haemolytica</I> A1 and A2 serotypes, grown under stress conditions using gentamycin during logarithmic growth. In this study 2 strains of <I>M. haemolytica</I> were used: Strain ATCC A1 and the second strain was the A2 serotype obtained from a pneumonic lung. Characterization of <I>M. haemolytica</I> MVs allows recognition of the main bacterial antigens in these structures, which makes them candidates for immunological evaluation in animal models.
ER  -