TY  - JOUR
T1  - Simultaneous Detection and Identification of Epizootic Hemorrhagic Disease Virus Serotype 1 and 2 using A Multiplex RT PCR
AU - , M.M. Salih AU - , S H. Idress AU - , S. M. M. Elamin AU - , R. A. Omer AU - , A. A. Majid AU - , A. E. Karrar AU - , M A. Abdalla AU - , M. E. H. Mohamed AU - , I. E. Aradaib 
JO  - Journal of Animal and Veterinary Advances
VL  - 2
IS  - 10
SP  - 585
EP  - 589
PY  - 2003
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2003.585.589
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2003.585.589
KW  - 
AB  - A multiplex reverse transcriptase (RT) polymerase chain reaction (RT-PCR)-based assay, for simultaneous serogroup-specific detection and serotype-specific identification of North American serotypes of epizootic hemorrhagic disease virus (EHDV) in cell culture and clinical samples, was developed. For detection of EHDV serogroup-specific, a pair of primers (EG1 & EG4) was designed from a conserve region of non-structural protein 1 (NS1) genome of EHDV serotype 2 (EHDV-2). For serotype-specific identification, two pairs of primers (ES1 and ES4) and (ESa and ESb) were designed from variable regions of genome segment 2 (L2) of EHDV-1 and that of EHDV-2. The multiplex RT-PCR-based assay utilized a single tube-PCR amplification in which EHDV serogroup-specific and serotype-specific primers were used simultaneously in a multiplex format. The EHDV serogroup-specific primers generated a 387 base pair (bp) PCR product from RNA samples of EHDV-1 and EHDV-2. The EHDV serotype-specific primers generated a 821-bp PCR product and a 1054-bp PCR product from RNA samples of EHDV-1 and EHDV-2, respectively. However, RNAs from BTV serotypes 2, 10, 11, 13 and 17; or total nucleic acid extract from non infected Vero cells failed to demonstrate the specific EHDV PCR products. The described multiplex RT-PCR-based assay could be used to facilitate rapid detection and differentiation of EHDV serotypes 1 and 2. In addition, it could also be used to monitor incursion of new serotypes of EHDV in North American continent
ER  -