@article{MAKHILLRJAS2014939349,
title = {Studies on in vitro Antioxidant Activity of Marine Edible Seaweed
From East Coastal Region, Peninsular Malaysia Using Different Extraction Methods},
journal = {Research Journal of Applied Sciences},
volume = {9},
number = {3},
pages = {141-146},
year = {2014},
issn = {1815-932x},
doi = {rjasci.2014.141.146},
url = {https://makhillpublications.co/view-article.php?issn=1815-932x&doi=rjasci.2014.141.146},
author = {Tam Siow,Lian Ai,Palaniselvam,Mashitah M. and},
keywords = {Edible seaweeds,phenolic compound,extraction,DPPH,FT-IR},
abstract = {In this present study the antioxidant activity of two edible
seaweeds Eucheuma cottonii and Padina sp. were determined. The
two extraction methods such as conventional and soxhlet were used to extract
secondary metabolites by using methanol as a solvent medium. Total phenolic
content of crude seaweed extracts were analysed by standard method. The different
antioxidant assays 2,2-Diphenyl-1-Picrylhydrazyl (DPPH), Ferric Reducing Antioxidant
Power (FRAP) and β-carotene bleaching assays were studied. The DPPH and
FRAP assays showed positive correlation with expressed higher total phenolic
content of methanolic seaweed extracts. Also, β-carotene bleaching assay
lower activity compare with BHT as reference control. IR spectra showed the
phenolic related functional groups are present the seaweed extract. It mainly
responsible for higher rate of antioxidant activity. The methanolic extracts
of Padina sp. showed better radical scavenging and higher phenolic content
than Eucheuma cottonii. Also, the soxhlet extraction showed higher yield
and better radical scavenging activity compared to conventional method. Moreover,
the studies confirmed both seaweeds are an effective candidate for the control
the free radical scavenging activity.}
}