@article{MAKHILLRJAS2010519007,
title = {Changes in Membrane Permeability During Rehydration of Freeze-Dried Weissella paramesenteroides LC11},
journal = {Research Journal of Applied Sciences},
volume = {5},
number = {1},
pages = {7-12},
year = {2010},
issn = {1815-932x},
doi = {rjasci.2010.7.12},
url = {https://makhillpublications.co/view-article.php?issn=1815-932x&doi=rjasci.2010.7.12},
author = {P.,K.M. and},
keywords = {lactic acid bacteria,freeze-drying,Viability,starter culture,membrane integrity,fermented foods},
abstract = {The objective of this study was to assess changes in membrane permeability during rehydration of freeze-dried Weissella paramesenteroides LC11. Viability was assessed using the electrical conductivity measurement (ms/cm/g dry weight) and the plate count method (cfu g-1 dry weight). The symptoms of injury included an increase in the electrolyte leakage during the first 4 h of rehydration in Milli Q water and a decrease in the survival rate, suggesting an increase in membrane permeability during dehydration. An increase in the temperature, NaCl or monosodium glutamate concentration and a decrease in H+ concentration resulted in an increase in the electrolyte leakage and a decrease in the survival rate. However, a decrease in the electrolyte leakage was observed with increasing glycerol, sucrose or maltodextrin and resulted in the maintenance of cell viability. Regression analysis showed that overall, there was a good correlation between the survival rate and electrolyte leakage (R2>0.90). Change in membrane permeability might lead to electrolyte leakage during rehydration and ultimately, cell death.}
}