@article{MAKHILLJAVA201413114352,
title = {Screening of 11 Pathogens in Fur Animal Basesd on Oligonucleotide Microarray},
journal = {Journal of Animal and Veterinary Advances},
volume = {13},
number = {11},
pages = {671-677},
year = {2014},
issn = {1680-5593},
doi = {javaa.2014.671.677},
url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2014.671.677},
author = {Chao,Song-Kai,Qin and},
keywords = {microarray,oligonucleotide,viruses,bacteria,Fur animal,infection},
abstract = {Brucella, E. coli O157, Staphylococcus aureus,
β-Streptococcus, Erysipelothrix rhusiopathiae, Pseudomonas aeruginosa
and Bovine viral diarrhea virus, Sheep pox virus, Goat pox virus, Bluetongue
virus, foot and mouth disease virus are common pathogens in fur animal. An oligonucleotide
microarray able to detect simultaneously the 6 bacteria and 5 viruses is reported
in the present study. The assay was highly specific for detecting the 6 bacteria
and 5 viruses in single or multiple infections and as few as 100 copies of specific
pathogens target fragments were detected successfully. The 320 archived samples
were tested by this assay and the results were 100% consistent with previous
results based on conventional PCR and sequencing. The assay is appropriate for
the screening of 11 pathogens infections in fur animal due to its high throughput,
low-cost, high specificity and sensitivity.}
}