@article{MAKHILLJAVA20131264084,
title = {Ovulation Rate and Embryo Yield of Dorper Sheep in Non-Breeding
Season under Different Superovulatory Protocols},
journal = {Journal of Animal and Veterinary Advances},
volume = {12},
number = {6},
pages = {726-730},
year = {2013},
issn = {1680-5593},
doi = {javaa.2013.726.730},
url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2013.726.730},
author = {S.,E. and},
keywords = {Embryo transfer,dorper sheep,embryo recovery rate,superovulation,non-breeding season},
abstract = {The response to superovulatory treatment and the embryo yield
of Dorper ewes in a non-breeding season were evaluated. Average 65 kg, weight,
3 years old donor ewes were inserted CIDR for 12 days and divided into four
superovulation treatment groups; Exp. I: 12 mL Folltropin+200 I.U. eCG (n =
25); Exp. II: 10 mL Folltropin+200 I.U. eCG (n = 20); Exp. III: 10 mL Folltropin+600
I.U. eCG (n = 25) and Exp. IV: 10 mL Folltropin+750 I.U. eCG (n = 10) with single
shot of FSH at CIDR removal. Donors were superovulated using FSH-p (total of
20 mg) (Folltropin-V; Vetrepharm, Canada) applied in six decreasing doses of
3, 2.5, 2, 2, 1.5, 1 mL i.m. starting 60 h before CIDR withdrawal. Donors underwent
intrauterine AI with fresh diluted semen (a minimum of 50x106 motile
sperm/each uterine horn) 40 h after CIDR removal and embryos collected 6 days
after insemination. The CL responses were found to be similar in all treatment
groups (Exp. I: 11.7±1.18; II: 9.7±1.31; III: 8.4±1.76
and IV: 9.9±1.63). Superovulation response (CL>3) was found relatively
but not significantly higher in lower dose of eCG treated Exp. I: 92% and II:
91%, respectively compared to III: 76% and IV: 75%. Transferable embryo numbers
for a fresh MOET program were found slightly higher (P: 0.333) in donors in
Exp. III with 7.8 ±1.2 than those recorded for donors in Exp. I (4.7±1.12),
II (6.3±1.24) and IV (6.3±1.7). The significant difference (p<0.05)
was observed in embryo recovery rates; Exp. II and III: 63%, Exp. I: 38% and
IV: 43%. Stages of embryonic development were also affected by the different
hormonal treatments used in the study Exp. I and IV giving the best results
for a fresh MOET program. In conclusion, it was found that Dorper ewes treated
with different superovulation protocols in out of season (May) in vivo
fresh embryo MOET program yielded 9 embryos and the recovery rate was markedly
higher than those of earlier findings. Study also showed that a single FSH injection
can be used in place of decreasing dose of multiple FSH injections with similar
results.}
}