@article{MAKHILLJAVA201312144206,
    title = {The Impact of Copper and Zinc Supplementation on Serum Haptoglobin and Milk Production Performance on 20 Weeks of Lactation in Dairy Cows},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {12},
    number = {14},
    pages = {1241-1249},
    year = {2013},
    issn = {1680-5593},
    doi = {javaa.2013.1241.1249},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2013.1241.1249},
    author = {Alina,Gheorghe and},
    keywords = {Copper,dairy cows,haptoglobin,zinc,sulphate},
    abstract = {The effect of copper and zinc supplementation on serum haptoglobin 
  and milk production performance was tested in a 20 weeks feeding trial with 
  40 Holstein-Friesian lactating cows, devided in 2 equal groups (supplemented 
  and control). At 10-20 days post calving cows began receiving either a diet 
  containing supplemental Cu and Zn sulphate (19.1±0.8 ppm Cu and 61.7±6.8 
  ppm Zn) or a diet without mineral supplement (3.1±0.4 ppm Cu, 9.2±1.1 
  ppm Zn). Milk and blood samples were collected six times every 28 days. Ten 
  subjects of each group were randomly assigned to skin biopsies of the periorbital 
  area at the end and the beginning of the study. Feeding inorganic Cu and Zn 
  increased (p<0.001) plasma Cu and Zn values and significant interactions 
  (p<0.001) was found between time and supplementation on plasma Cu and Zn 
  levels. Serum haptoglobin values were lower (p = 0.24) in cows from supplemented 
  group (0.096&plusmn;0.006 g L<SUP>-1</SUP>) relative to the control group (0.099&plusmn;0.009 
  g L<SUP>-1</SUP>). There was no effect of time (p = 0.21) and no significant 
  interaction (p = 0.35) between time and supplementation on serum haptoglobin 
  levels. The Body Condition Score (BCS) was higher in supplemented group than 
  in those that did not receive supplemental minerals (2.82 vs. 2.75, p = 0.03) 
  but no significant interaction was found between time and supplementation on 
  BCS (p = 0.85). Feeding inorganic Cu and Zn reduced periorbital hypopigmentation 
  in cows. Supplementing Cu and Zn comparing to the control cows had higher yield 
  of milk (23 vs. 22 kg day<SUP>-1</SUP>, p = 0.004), milk crude protein (3.3 
  vs. 3.2%, p = 0.14), milk fat (4.16 vs. 4.09%, p = 0.002), milk lactose (4.82 
  vs. 4.79 g kg<SUP>-1</SUP>, p = 0.32) and milk energy (0.74 vs. 0.75 Mcal kg<SUP>-1</SUP>, 
  p = 0.27) however, the response to supplementation tended to be inconsistent 
  over time for production indicators as indicated by time x supplementation interactions 
  (p&gt;0.15). Supplemented group had lower SCC compared to the control cows (246,000 
  vs. 288,000 cells mL<SUP>-1</SUP>, p = 0.02) but no significant interaction 
  was found between time and supplementation on SCC (p = 0.47). Supplementing 
  with 1.5 g CuSO<SUB>4</SUB>A5H<SUB>2</SUB>O and 5 g ZnSO<SUB>4</SUB>A7H<SUB>2</SUB>O/cow/week 
  for 1st 20 weeks of lactation can significant improve health and milk production 
  performances in a deficient area.}
    }