@article{MAKHILLJAVA201211243915,
title = {Analysis of the Effect of Virulent Mareks Disease Virus SORF2
Gene on its Horizontal Transmission Capacity Using Real-Time PCR},
journal = {Journal of Animal and Veterinary Advances},
volume = {11},
number = {24},
pages = {4648-4654},
year = {2012},
issn = {1680-5593},
doi = {javaa.2012.4648.4654},
url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.4648.4654},
author = {Shuai,Ning,Yixin,Aijun,Pengfei,Zimeng,Peng and},
keywords = {Marek`s disease virus,SORF2 gene,horizontal transmission capacity,real-time PCR,China},
abstract = {Mareks Disease Virus (MDV) Chinese strain GX0101 was
the first reported recombinant MDV field strain with one Reticuloendotheliosis
Virus (REV) Long Terminal Repeat (LTR) insert. REV LTR in the GX0101 genome
increased the potential for horizontal transmission. REV LTR fragment has the
biggest impact on SORF2 gene. Researchers detected the influence of SORF2
gene deletion on its horizontal transmission ability by real time PCR. The result
shows that GX0101ΔSorf2 possesses similar replication ability with bac-GX0101
no mater on the level of lymphocyte or feather follicle. Although, researchers
can detect MDV positive in feather follicles of sentinel in contact chickens
at age 10 in both groups respectively, the number of SPF chickens infected with
bac-GX0101 by contact is higher than the number of GX0101ΔSorf2 infection
group. Therefore, SORF2 gene has certain effect on the horizontal transmission
ability of the virus.}
}