@article{MAKHILLJAVA201211203816,
title = {Characterization of β-Actin Promoter of Leuciscus merzbacheri},
journal = {Journal of Animal and Veterinary Advances},
volume = {11},
number = {20},
pages = {3849-3854},
year = {2012},
issn = {1680-5593},
doi = {javaa.2012.3849.3854},
url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.3849.3854},
author = {Shiwei,Wenge,Chuangfu,Yuanzhi,Yan,Xudong and},
keywords = {Leuciscus merzbacheri,promoter analysis,B-actin gene,green fluorescent protein,China},
abstract = {Leuciscus merzbacheri is a unique vulnerable indigenous
fish which is only distributed in the Junggar Basin, Xinjiang. In this study,
researchers cloned two L. merzbacheri β-actin promoter fragments
of different length: SZ11 and SZ21 and analyzed their structural features. The
mammalian expression vector pEGFP-N1 was used to construct eukaryotic expression
vectors β1 pEGFP-N1-AFP III and β2 pEGFP-N1-AFP III in which fish
type III antifreeze protein gene was used as the structural gene. The results
showed that the cloned two fragments of β-actin promoters had the ability
to drive the expression of the green fluorescent protein gene in BHK-21 cells.
These data suggest that the vectors researchers constructed based on β-actin
promoter could be exploited as all fish recombinant eukaryotic expression vector.}
}