@article{MAKHILLJAVA20121173337,
    title = {Prokaryotic Expression and Sequence Analysis of Porcine <I>BCL10</I> Gene},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {11},
    number = {7},
    pages = {1046-1051},
    year = {2012},
    issn = {1680-5593},
    doi = {javaa.2012.1046.1051},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.1046.1051},
    author = {Wanzhu,Zhicai,Shan,Xinqiao,Yue,Zhiwen,Ling and},
    keywords = {BCL-10,molecular cloning,Bioinformatics,Prokaryotic expression,human,China},
    abstract = {BCL-10 is an important functional and an apoptotic regulator gene inducing the organismic innate immunity. It has not been reported that the BCL-10 protein of porcine in normal level could motivate immune factor to produce. Bioinformatics analysis was performed to predict the characteristics of BCL-10, the results indicated that <I>BCL-10</I> gene encoded a polypeptide, molecular mass of 45 Ku and comprising 233 amino acids. The prediction of secondary structure showed that BCL-10 had 9 alpha-helix, 1 beta-sheet, 12 turn and 14 random coil. The protein had twenty transmembrane regions, an CARD structure field which located in 15-100 amino acids and no signal peptide, the hydrophobic index was between -2.500 and 1.400. The phylogenetic tree showed that BCL-10 was similar to human (the homology index was &gt;95%) and had a distant relationship to mouse. To identify the activity of BCL-10, the protein with a His-tag was expressed in <I>E. coli</I> Rosetta and purified. The purified protein showed an obvious effect of cell proliferation. Cloning and analysising of <I>BCL-10</I> gene which laid foundation to further research and exploitation.}
    }