@article{MAKHILLJAVA200652238, title = {Effects of Soluble and Insoluble Non-starch Polysaccharides Isolated from Wheat Bran on Endogenous Amino Acid Loss at the Terminal Ileum of Growing Rats}, journal = {Journal of Animal and Veterinary Advances}, volume = {5}, number = {2}, pages = {143-149}, year = {2006}, issn = {1680-5593}, doi = {javaa.2006.143.149}, url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2006.143.149}, author = {Y.H. He,W.Q. Lu,D.F. Li,H.L. Zhang and}, keywords = {Ileal endogenous amino acid loss,wheat,non-starch polysaccharides,rat}, abstract = {The objective of this study was to investigate the effects of soluble and insoluble non-starch polysaccharides isolated from wheat bran on endogenous amino acid flow at the terminal ileum of growing rats using the protein-free method. In experiment 1, 20 Sprague-Dawley rats (200 ± 0.9 g), were fed either a protein-free, low fiber diet based on cornstarch, sucrose and soybean oil or a similar diet containing 50 g kg 1 of soluble non-starch polysaccharides added at the expense of cornstarch. In experiment 2, 40 Sprague-Dawley rats (198 ± 0.8 g), were randomly allotted to either the protein-free, low fiber diet or similar diets containing either 50 g kg 1 insoluble non-starch polysaccharides, 50 g kg 1 soluble non-starch polysaccharides or a combination of 50 g kg 1 soluble and 50 g kg 1 insoluble non-starch polysaccharides added at the expense of cornstarch. Chromic oxide (6 g kg 1 diet) was included in all diets as an indigestible marker. Both experiments lasted 8 d. The rats were killed on day 8 and the digesta contained in the final 20 cm of the ileum was obtained for assay. Compared with the protein-free basal diet, adding 50 g kg 1 soluble non-starch polysaccharides significantly (p<0.01) increased the endogenous flow of amino acids and nitrogen at the terminal ileum (both experiment 1 and 2). Including 50 g kg 1 of insoluble non-starch polysaccharides also significantly increased (p<0.05) the ileal flows of amino acids and nitrogen. The effects of the soluble non-starch polysaccharides were 22-85% greater than those of the insoluble non-starch polysaccharides.} }