@article{MAKHILLJAVA20032121559,
    title = {Application of RT-PCR for Detection of African Isolates of Palyam Orbiviruses Serogroup in Cell Culture},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {2},
    number = {12},
    pages = {671-674},
    year = {2003},
    issn = {1680-5593},
    doi = {javaa.2003.671.674},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2003.671.674},
    author = {Abdel Rahim E. Karrar,Mohamed A. Abdalla,Ali M. Majid and},
    keywords = {},
    abstract = {A reverse transcriptase (RT) polymerase chain reaction (RT-PCR)-based assay, for detection of African isolates of palyam virus ribonucleic acid (RNA) in cell culture, was developed. A pair of oligoribonucleotide primers (pal1 and pal2), selected from genome segment 3 of Chuzan virus, an isolate of the palyam serogroup, was used as a target for PCR amplification. Using RT-PCR, the pair of primers (pal1 and pal2) resulted in amplification of a 660-bp product. RNA samples from African isolates of palyam virus serogroup, propagated in cell cultures, were detected by this RT-PCR-based assay. Amplification product was not detected when the palyam RT-PCR-based assay was applied to RNA from, closely related orbiviruses, bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV); total nucleic acid extracts from uninfected Vero cells. The described RT-PCR-based assay provides a rapid, sensitive and specific method for detection and differentiation of palyam serogroup of orbiviruses in cell culture.}
    }