Zuo-Hua  Liu, Rui-  Yang, Ke-Fei  Shen, Kang-Cheng  Pan, Ming-  Wen, Jin-Long  Yang, 
    Protective Effects of Dietary Co-Administration of Probiotic<I> Lactobacillus 
  casei</I> on CP-Induced Reproductive Dysfunction in Adult Male Kunming Mice,
    Veterinary Research,
    Volume 5,Issue 5,
    2012,
    Pages 110-119,
    ISSN 1993-5412,
    vr.2012.110.119,
    (https://makhillpublications.co/view-article.php?doi=vr.2012.110.119)
    Abstract: Cyclophosphamide (CP), an anti-cancer alkylating agent is 
  known to be a male reproductive tract toxin. The aim of this study was to investigate 
  the possible protective effects of <I>Lactobacillus casei</I> as a probiotic 
  product and potent antioxidant on CP-induced testicular and spermatozoal toxicity 
  associated with oxidative stress in male mice. Seventy two healthy adult male 
  Kunming mice were divided into eight groups of nine mice each. The blank group 
  (P1) and the CP Model group (P2) were fed the basal pellet diet without any 
  probiotics. The mice in the <I>Lactobacillus casei</I>+CP groups P3, P4, P5 
  and P6 were fed the basal pellet diet with 10<SUP>5</SUP>, 10<SUP>6</SUP>, 10<SUP>7</SUP>, 
  10<SUP>8</SUP> CFU g<SUP>-1</SUP> (diet) of <I>Lactobacillus casei</I>. The 
  P7 and P8 group mice administered with <I>Lactobacillus casei</I> but not CP 
  as a control group. For the 1st 5 days, mice in the P2, P3, P4, P5 and P6 groups 
  were injected intraperitoneally with CP. All treatments were maintained for 
  8 weeks. At the end of the treatment period, body weight and the weight of the 
  reproductive organs was determined. Histological and biochemical analyses of 
  sperm and testicular tissue and serum sex hormone levels were performed. Administration 
  of CP resulted in significant decreases in epididymal sperm concentration and 
  motility and significant increases in the number of abnormal sperm and Malondialdehyde 
  (MDA) and Catalase (CAT) levels. However, <I>Lactobacillus casei</I> co-treatment 
  prevented the development of CP-induced sperm and testicular damage. Compared 
  with the CP groups, the <I>Lactobacillus casei</I>-treated groups showed increases 
  in testicular and epididymal weights, Superoxide Dismutase (SOD) and sperm concentrations, 
  percentages of morphological normality and serum testosterone levels. No significant 
  differences were observed in the number of abnormal sperm in the epididymides 
  and histomorphology of the testes between the P4, P5, P6, P7 and P8 groups and 
  blank group. <I>Lactobacillus casei</I> has a significant protective effect 
  on CP-induced toxicity in the male mice reproductive system.
    Keywords: reproductive dysfunction;antioxidant;Lactobacillus casei;probiotic;Cyclophosphamide