M.Z.  Wang, L.H.  Yu, H.R.  Wang, X.  Liu, 
    Effects of Forage Level in Diet on Bacterial Protein Recycling in Goat Rumen,
    Research Journal of Animal Sciences,
    Volume 4,Issue 1,
    2010,
    Pages 10-15,
    ISSN 1993-5269,
    rjnasci.2010.10.15,
    (https://makhillpublications.co/view-article.php?doi=rjnasci.2010.10.15)
    Abstract: The objective of this study was to determine the effects of forage level on bacterial protein recycling in the rumen by Fluorescence-Labeled Bacteria technique (FLB). Four Xuhuai goats with permanent cannulas were used in a 4x4 Latin squares and diets were divided into A (86.96), B (67.63), C (48.31) and D (28.99%) on the basis of forage level in diet. The results showed that rumen protozoal and bacterial populations were shifted heavily by diets (p<0.01) and C recorded the highest protozoal density while A demonstrated the lowest densities of protozoa and bacteria. Significant differences were detected in protozoa engulfing rates between groups (p<0.01) with the lowest value dropping in B. It was also observed that C had the highest bacterial turnover rate and the shortest recycling time. Further estimation of bacterial protein recycling quantity per day per goat resulting from protozoa predation showed that C interpreted the highest bacterial protein recycling quantity while B had a lower bacterial protein recycling quantity, accounted for just 68.2% of that of group C. Results obtained here indicated that diets containing 67.63% forage could curb the bacterial protein recycling in some degree by regulating protozoal biomass and their ingestion action.
    Keywords: Forage level;rumen;protozoa;bacteria;engulfing rate;protein recycling;China