J. Isaac Joshua Ramesh Lalvani, B. Dhinesh, M. Parthasarathy, Habtewolde Ababu, Collective Effect of Mutated Piston and Different Injection Timing on Biodiesel Mixed Diesel Fuel in Diesel Engine, Journal of Engineering and Applied Sciences, Volume 15,Issue 8, 2020, Pages 1894-1903, ISSN 1816-949x, jeasci.2020.1894.1903, (https://makhillpublications.co/view-article.php?doi=jeasci.2020.1894.1903) Abstract: The commercial development of all nations builds upon the provision of energy. Sustained energy supply is extremely important for a developing country. Emerging awareness of air pollution and together with global warming is alarming us and making us to critically think about an alternative source of ecofriendly supply of energy. Thus, serious efforts are being done to produce new forms of fuels that can successfully replace the naturally occurring and rapid exhausting fuel. In this research, engine energized with non-edible vegetable oil ester blend is studied. Engine studies have been carried out using standard diesel and 20% mixture of Adelfa biodiesel [AOME20]. Data acquired from sustainable fuel AOME20 in the engine showed the reduction in thermal efficiency due to deprived air fuel mixing features and its superior viscosity. This urged the studies towards the furtherance of air fuel mixing by a novel Mutated Piston [MP]. Further studies were aimed towards the study of the collective effect of mutated piston and injection timing. About 1.23% progress in performance of engine energized with non-edible vegetable oil ester blend was obtained for retarded injection timing of 21°bTDC, as compared with the standard injection timing 21°bTDC. Hydro carbon, carbon monoxide and smoke intensity were diminished significantly for Mutated Piston [MP] with AOME20 with retarding injection timing whereas nitrous oxide and carbon dioxide emissions were elevated. Keywords: mutated piston;combustion improvement;combustion chamber;biodiesel;injection timing;Air movement inducement