Xiaobo  Zheng, Rong  Xiao, Jiao  Li, Zhiwan  Zheng, Lijuan  Qing, Libin  Wang, Yinzhu  Yao, Boqing  Wu, Huihao  Xu, Maosen  Xu, Jing  Li, Qun  Wu, Liang  Ge, 
    The Effect of C-Jun on Leydig Cells Proliferation of Elemental Status in Rongchang Piglets,
    Agricultural Journal,
    Volume 8,Issue 5,
    2013,
    Pages 256-261,
    ISSN 1816-9155,
    aj.2013.256.261,
    (https://makhillpublications.co/view-article.php?doi=aj.2013.256.261)
    Abstract: Leydig cells were isolated from the testis of 3 week old Rongchang piglets. Its activity, purity and function were qualified before being cultured <I>in vitro</I>. Then, they were respectively treated with 0.125, 0.25, 0.5, 1 and 2 &#956;mol L<SUP>-1</SUP> c-jun ASODNs and the expression of c-jun mRNA were detected by FQ-PCR. To study the effect of c-jun on proliferation of elemental status leydig cells in piglets, the leydig cells had been treatedwith c-jun ASODNs and the growth inhibition ratio of leydig cells were detected by MTT method and cell cycle phase distribution were examined by flow cytometer analysis. The FQ-PCR assays results indicated that c-jun mRNA expression decreased with the rise of c-jun ASODNs concentration. Only 0.125 &#956;mol L<SUP>-1</SUP> c-jun ASODNs could reduce the expression of <I>c-jun</I> gene significantly (p&lt;0.01). And the expression no longer went down when the concentration was up to 1 &#956;mol L<SUP>-1</SUP>. Through MTT analysis, it is found that c-junASODNs could inhibit the growth of leydig cells significantly (p&lt;0.01). There is a positive correlation between c-jun ASODNs concentration and growth inhibition ratio of leydig cells (r = 0.8966).
    Keywords: C-jun;piglet;leydig cells;proliferate;China