Zhang  Jing-Hu, Gong  Wei-Hua, Yang  Hong-Wen, 
    Isolation, Sequence Identification and Tissue Expression Profile of a Novel Goat Gene <I>RHOA</I>,
    Agricultural Journal,
    Volume 7,Issue 3,
    2012,
    Pages 194-197,
    ISSN 1816-9155,
    aj.2012.194.197,
    (https://makhillpublications.co/view-article.php?doi=aj.2012.194.197)
    Abstract: The full-length cDNA sequence of one goat gene <I>RHOA</I> was amplified using the rapid amplification of cDNA ends (RACE) method based on one goat EST sequence which was highly homologous to the coding sequence of human <I>RHOA</I> gene. Sequence prediction analysis revealed that the open reading frame of this gene encodes a protein of 193 amino acids that shares high homology with the ras homolog gene family, member A (<I>RHOA</I>) of nine species human (100%), cattle (100%), sheep (100%), rat (99%), mouse (99%), chicken (99%), dog (99%), turkey (99%) and green anole (99%) so that it can be defined as goat <I>RHOA</I> gene. This novel goat gene was then deposited into NCBI database. Phylogenetic analysis revealed that the goat <I>RHOA</I> gene has a closer genetic relationship with the <I>RHOA</I> genes of human, cattle and sheep. Tissue expression analysis indicated that the goat <I>RHOA</I> gene is generally and differentially expressed in detected tissues including spleen, muscle, skin, kidney, lung, liver, fat and heart. This experiment established the primary foundation for further research on the goat <I>RHOA</I> gene.
    Keywords: Goat;RHOA;RACE;tissue expression profile;sequence identification;China