P.A. Egwaikhide  , S.O. Okeniyi  , E.E Akporhonor  , S.O. Emua  , 
    Studies on Bioactive Metabolites Constituents and Antimicrobial Evaluation of Leaf Extracts of Eucalyptus Globulus,
    Agricultural Journal,
    Volume 3,Issue 1,
    2008,
    Pages 42-45,
    ISSN 1816-9155,
    aj.2008.42.45,
    (https://makhillpublications.co/view-article.php?doi=aj.2008.42.45)
    Abstract: Hexane, ethyl acetate and methanolic extracts of dried powdered leaves of Eucalyptus globulus were screened for basic secondary metabolites and antibacterial activity against <I>C. pyogenes</I>, <I>S. aureus</I>, <I>S. faecallis</I>, <I>B.  stearothermophilus</I>, <I> S.  epidermidis</I>, <I>B. cereus</I>, <I>B. polymyxa</I>, <I>K. pneumonia</I>, <I>P. aeruginosa</I>, <I>B. anthacis</I>, <I>B. subtilis</I>, <I>E. coli</I>, <I>P. fluorescens</I> and <I>C. sporogenes</I>. Phytochemical investigation of crude extracts of Eucalyptus globulus (leaf) revealed the presence of tannins, phlobatannins, saponins, flavonoids, steroids, terpenoids, cardiac glycosides and reducing sugar in the plant. The antimicrobial sensitivity of the extracts against  four  species  of  Gram-ve  and  ten  Gram+ve  bacterial stain for Eucalyptus globulus (leaf) revealed very high antimicrobial activity. The extracts of hexane, ethylacetate and methamol of Eucalyptus globulus (leaf) were all very active on almost all the bacterial strains. The Infra Red (IR) spectra revealed the presence of different functional groups ranging from OH (3406-3338.6 cm <SUP>1</SUP>) stretching, C-H (2926.6 cm <SUP>1</SUP>, 864.1 cm <SUP>1</SUP>) stretching   alkyl,    methyl    C = 0, (2162.1 cm )   anhydrides,   C = C (1697.6 cm <SUP>1</SUP>)   aromatic   ring  sretching, C-0 (1310.6-1059 cm <SUP>1</SUP>) alcohol, ethers, esters, carboxalic acid bending.
    Keywords: Bioactive metabolites;antimicrobial evaluation;eucalyptus;globules;phytochemical screening;bacterial strain;bacterial strain;functional groups