Yong Zhang  , Ya Luo  , Yan-Xia Hou  , Hao Jiang  , Qing Chen  , Hao-Ru Tang  , 
    Chilling Acclimation Induced Changes in the Distribution of H<SUB>2</SUB>O<SUB>2 </SUB>and Antioxidant System of Strawberry Leaves,
    Agricultural Journal,
    Volume 3,Issue 4,
    2008,
    Pages 286-291,
    ISSN 1816-9155,
    aj.2008.286.291,
    (https://makhillpublications.co/view-article.php?doi=aj.2008.286.291)
    Abstract: Changes in the contents of H<SUB>2</SUB>O<SUB>2</SUB>, malondialdehyde (MDA) and endogenous antioxidants, the activities of enzymes involving cell defense in leaves of strawberry (<I>Fragaria</I>×<I>Ananassa</I> Duch.) plantlets under chilling acclimation and de-acclimation were studied. With the increase of chilling acclimation times, the contents of H<SUB>2</SUB>O<SUB>2</SUB> and MDA increased to a certain degree and then decreased. The activities of cell defence enzymes, such as Superoxide Dismutase (SOD), catalase (CAT), peroxidase (POD), Dehydroascorbate Reductase (DHAR), Monodehydroascorbate Reductase (MDAR), Ascorbate Peroxidase (APX) and glutathione reductase (GR) were gradually increased to a certain degree and then kept at a high level. And it increased the contents of reduced ascorbate (ASA), dehydroascorbate (DHA) and reduced glutathione (GSH). Our results clearly suggested that cold-hardening triggers an increase of reactive oxygen species (ROS) and the early accumulation of ROS in plants might lead to the production of antioxidant defense system. Based on the obtained results, it can be concluded that the ascorbate-glutathione (ASA-GSH) cycle plays an impotant role in enhancement of chilling resistance of strawberry during chilling acclimation.
    Keywords: Fragaria×Ananassa Duch;chilling acclimation;antioxidant system;ASA-GSH cycle