Solomon  Yeshanew, Yalemtsehay  Mekonnen, 
    The Effect of <I>Otostegia integrefolia</I> Leaf Extracts on the Packed 
  Cell Volume, Body Weight and Survival Time of <I>Plasmodium berghei</I> Infected Mice,
    International Journal of Tropical Medicine,
    Volume 8,Issue 5,
    2013,
    Pages 129-134,
    ISSN 1816-3319,
    ijtmed.2013.129.134,
    (https://makhillpublications.co/view-article.php?doi=ijtmed.2013.129.134)
    Abstract: The antimalarial suppressive test of <I>Otostegia integrefolia</I> 
  leaf extracts previously demonstrated and showed promising results in mice. 
  The present study is aimed at evaluating the protective capability of the crude 
  extracts of the leaves of the plant against loss of body weight, reduction in 
  Packed Cell Volume (PCV) and shortening survival time caused by the malaria 
  parasite using <I>Plasmodium berghei</I> infected mice. A standard 4 days Peter&#146;s 
  antimalarial test was carried out. PCV and body weight of mice were measured 
  before parasite inoculation and after 4 days successive treatment. Starting 
  from parasite inoculation up to death, survival time of each mouse in each group 
  was also recorded. Aqueous, methanol and chloroform crude extracts of the plant 
  material at tested doses of 200, 400 and 800 mg kg<SUP>-1</SUP> were administered 
  orally for the treatment and vehicles for the control groups. The result showed 
  that, extracts of the plant material were able to prevent significant (p&lt;0.05) 
  loss of body weight and reduction in the PCV of mice. Similarly, aqueous and 
  methanol extract treated mice lived longer survival time than the respective 
  negative controls. Thus, polar solvent extracts of the plant material are able 
  to reduce the overall pathogenic effects of the <I>Plasmodium berghei</I> parasitaemia 
  in mice.
    Keywords: Body weight;in vivo;Otostegia integrefolia;packed cell volume;sub-acute;survival time