Sadanand Fulzele  , Raj Kumar Singh  , Madhusudan Hosamani  , Bimalendu Mondal  , Mahendra Pal Yadav  , 
    Evaluation of Duplex PCR and PCR-RFLP for Diagnosis of Sheep Pox and Goat Pox,
    International Journal of Tropical Medicine,
    Volume 1,Issue 2,
    2006,
    Pages 66-70,
    ISSN 1816-3319,
    ijtmed.2006.66.70,
    (https://makhillpublications.co/view-article.php?doi=ijtmed.2006.66.70)
    Abstract: In the present study, we evaluated the diagnostic efficacy of attachment gene based PCR (A-PCR)
and A-PCR-RFLP employing a number of <I>capripoxvirus </I>field samples received from different parts of the
country during 2000-2005. The tests were compared with duplex PCR and A-PCR-RFLP methods. We also
cloned and sequenced A-PCR products from three representative Goatpoxvirus (GPV) isolates and three
representatives Sheeppox Virus (SPV) isolates. F-PCR products of two isolates of GPV were also cloned and
sequenced. Sequence variation in the attachment and fusion gene fragments of SPV and GPV was also
determined to confirm the differential amplification in the suspected clinical samples. Phylognetic analysis
based on partial fusion gene sequence of capripoxviruses showed that Indian isolates are closely related to
other GPV strains published earlier.
    Keywords: Goat pox;sheep pox;diagnosis;PCR